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Lab Protocols:
Below is a list of useful information and protocols for the Langerhans Lab. Remember to use the Lab Google Calendar to schedule the use of any major equipment (e.g., cameras, x-ray machine, microscope) or vehicle.
Contact Info and Directions: - Location and directions to the lab and Dr. Langerhans’ office - Useful Phone Numbers - Mailing Address
DRILL Protocols and Templates: - DRILL Overview - DRILL Processing and Photographing - DRILL Photo Template - DRILL Paper Label Template - X-Ray Template Protocol - DRILL X-Ray Template - Live Fish Photography Protocol - Checklist for Adding a Collection to the DRILL - DRILL Admin and Updating the Web Server - Updating DRILL with txt File - DRILL Data and Server Backup - Convert Lat/Long to Decimals
Molecular Protocols: - Agarose Gel Electrophoresis - DNA Concentration Quantification - DNA Oligonucleotide Resuspension and Storage - PCR Primers - PCR Protocols - Sample Prep for Genewiz
General Information: - Major Equipment List - Camera Equipment - Chemical Waste Disposal - Ethanol Dilution Protocol - FTP Data Backup - Lab Truck Protocol - Shipping from NCSU - Receiving Foramlin-Preserved Specimens - Vendor List
Live Fish Care: - Live Fish Care Overview (out of date) - Live Fish Care Daily Checklist - Artemia Culture - BRF 223 Rack Maintenance - Water Chemistry Target Ranges - Water Treatment & Aging - 5-20 Gallon Tank Maintenance - Aquaria Water Changes - Handling Dead Fish at Yates Mill - Yates Mill Light Timer Programming - Live Fish Importation-Bahamas
Lab Techniques / Data Collection: - X-Ray Imaging - Gonopodium Tip Imaging - Landmark Acquisition for GeoMorph - High-speed Video Overview - Life History Dissection Protocol - Common Statistical Methods - R scripts - Detecting Prey Items with X-rays - Overview of Gambusia Prey Items
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